Coding

Part:BBa_K2915150:Design

Designed by: SAADOUNI Rim   Group: iGEM19_Aix-Marseille   (2019-10-11)


IPTG inducible promoter (T7) with RBS AFP-lu1 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a 6Hig-Tag onto the "AFP protein" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f APF protein in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.


Source

Nucleotide sequence

References